potatoes: source of laboratory reagent for human platelets research

Potatoes: Source of Laboratory Reagent for Human Platelets Research

Irish potatoes is rich in carbohydrate, with varied glucose levels. It is also a very good source of apyrase, an enzyme used for platelet storage. The aim of this study was to explore Irish potatoes as an easily available source for apyrase extraction and purification. Apyrase was extracted from 5 Kg of Irish potatoes. Platelets were derived from 5-10 ml of human whole blood and G6PD activity w...

The source of HLA molecules on platelets: Does platelets adsorb soluble HLA molecules from their environment?

Background: The origin and function of human leukocyte antigen (HLA) class I molecules on platelets are still highly arguable. Given the differences in the results of the previous studies in this regard, the lack of research in recent years, and the clinical importance of HLA class I molecules, the absorption capacity of platelets for soluble HLA class I molecules was studied in this investigat...

Laboratory evaluation of platelets for transfusion.

Induction of apoptosis in human tumor cell lines by platelets

Introduction: It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown. Materials and methods: To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thromb...

Development of dry reagent chemistry for the clinical laboratory

Conclusions It appears that on balance, these two systems are similar in terms of advantages and disadvantages. Each seems to have emphasised the more important characteristic for its potential application. Thus, the multilayer film tests seem to be being directed to large laboratories where high precision is important and storageability less so. The impregnated fibre systems, on the other hand...

Laboratory animal: biological reagent or living being?

The duties of humans toward non-human animals and their rights in society have been debated for a long time. However, a discussion on the terminology used for the identification of laboratory animals is usually not considered, although the employment of inadequate terminology may generate disastrous consequences for the animals before, during, and after the experiment. This study intends to def...